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Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection
Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection
- Source :
- Theoretical and Applied Genetics. 131:127-144
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Thirteen potentially new leaf rust resistance loci were identified in a Vavilov wheat diversity panel. We demonstrated the potential of allele stacking to strengthen resistance against this important pathogen. Leaf rust (LR) caused by Puccinia triticina is an important disease of wheat (Triticum aestivum L.), and the deployment of genetically resistant cultivars is the most viable strategy to minimise yield losses. In this study, we evaluated a diversity panel of 295 bread wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources (St Petersburg, Russia) for LR resistance and performed genome-wide association studies (GWAS) using 10,748 polymorphic DArT-seq markers. The diversity panel was evaluated at seedling and adult plant growth stages using three P. triticina pathotypes prevalent in Australia. GWAS was applied to 11 phenotypic data sets which identified a total of 52 significant marker–trait associations representing 31 quantitative trait loci (QTL). Among them, 29 QTL were associated with adult plant resistance (APR). Of the 31 QTL, 13 were considered potentially new loci, whereas 4 co-located with previously catalogued Lr genes and 14 aligned to regions reported in other GWAS and genomic prediction studies. One seedling LR resistance QTL located on chromosome 3A showed pronounced levels of linkage disequilibrium among markers (r 2 = 0.7), suggested a high allelic fixation. Subsequent haplotype analysis for this region found seven haplotype variants, of which two were strongly associated with LR resistance at seedling stage. Similarly, analysis of an APR QTL on chromosome 7B revealed 22 variants, of which 4 were associated with resistance at the adult plant stage. Furthermore, most of the tested lines in the diversity panel carried 10 or more combined resistance-associated marker alleles, highlighting the potential of allele stacking for long-lasting resistance.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Linkage disequilibrium
Quantitative Trait Loci
Plant disease resistance
Biology
Quantitative trait locus
Genes, Plant
01 natural sciences
Linkage Disequilibrium
03 medical and health sciences
Genetic variation
Botany
Genetics
Allele
Alleles
Genetic Association Studies
Triticum
Disease Resistance
Plant Diseases
Genetic association
Basidiomycota
Haplotype
Australia
Genetic Variation
food and beverages
General Medicine
Fixation (population genetics)
Phenotype
030104 developmental biology
Haplotypes
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 14322242 and 00405752
- Volume :
- 131
- Database :
- OpenAIRE
- Journal :
- Theoretical and Applied Genetics
- Accession number :
- edsair.doi.dedup.....642c2f8e5c50909c7599c2e2c7525df4
- Full Text :
- https://doi.org/10.1007/s00122-017-2990-5