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Separation and Identification of Isomeric Glycans by Selected Accumulation-Trapped Ion Mobility Spectrometry-Electron Activated Dissociation Tandem Mass Spectrometry
- Source :
- Analytical Chemistry. 88:3440-3443
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- One of the major challenges in structural characterization of oligosaccharides is the presence of many structural isomers in most naturally occurring glycan mixtures. Although ion mobility spectrometry (IMS) has shown great promise in glycan isomer separation, conventional IMS separation occurs on the millisecond time scale, largely restricting its implementation to fast time-of-flight (TOF) analyzers which often lack the capability to perform electron activated dissociation (ExD) tandem MS analysis and the resolving power needed to resolve isobaric fragments. The recent development of trapped ion mobility spectrometry (TIMS) provides a promising new tool that offers high mobility resolution and compatibility with high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometers when operated under the selected accumulation-TIMS (SA-TIMS) mode. Here, we present our initial results on the application of SA-TIMS-ExD-FTICR MS to the separation and identification of glycan linkage isomers.
- Subjects :
- Glycan
Chromatography
Fourier Analysis
biology
Ion-mobility spectrometry
Chemistry
010401 analytical chemistry
Analytical chemistry
010402 general chemistry
Top-down proteomics
Tandem mass spectrometry
Mass spectrometry
01 natural sciences
Article
Mass Spectrometry
Dissociation (chemistry)
Fourier transform ion cyclotron resonance
0104 chemical sciences
Analytical Chemistry
Isomerism
Polysaccharides
biology.protein
Structural isomer
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....642d998e2d65759edb7471f1172b5a4b