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BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma
- Publication Year :
- 2002
- Publisher :
- The National Academy of Sciences, 2002.
-
Abstract
- CTCF, a conserved, ubiquitous, and highly versatile 11-zinc-finger factor involved in various aspects of gene regulation, forms methylation-sensitive insulators that regulate X chromosome inactivation and expression of imprinted genes. We document here the existence of a paralogous gene with the same exons encoding the 11-zinc-finger domain as mammalian CTCF genes and thus the same DNA-binding potential, but with distinct amino and carboxy termini. We named this gene BORIS for B rother o f the R egulator of I mprinted S ites. BORIS is present only in the testis, and expressed in a mutually exclusive manner with CTCF during male germ cell development. We show here that erasure of methylation marks during male germ-line development is associated with dramatic up-regulation of BORIS and down-regulation of CTCF expression. Because BORIS bears the same DNA-binding domain that CTCF employs for recognition of methylation marks in soma, BORIS is a candidate protein for the elusive epigenetic reprogramming factor acting in the male germ line.
- Subjects :
- Genetic Markers
Male
CCCTC-Binding Factor
Molecular Sequence Data
Gene Expression
Paralogous Gene
Biology
X-inactivation
Genomic Imprinting
Mice
Testis
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Regulation of gene expression
Zinc finger
Genetics
Multidisciplinary
Sequence Homology, Amino Acid
Zinc Fingers
Biological Sciences
DNA Methylation
Protein Structure, Tertiary
DNA-Binding Proteins
Repressor Proteins
CTCF
DNA methylation
Genomic imprinting
Reprogramming
Transcription Factors
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....6438bc128662b2751f3b6373ff0d6f1b