Back to Search
Start Over
Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous cell carcinoma
- Source :
- PLoS ONE, Vol 8, Iss 4, p e62175 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Penile Squamous Cell Carcinoma (SCC) is a rare cancer with poor prognosis and limited response to conventional chemotherapy. The genetic and epigenetic alterations of Epidermal Growth Factor Receptor (EGFR)-RAS-RAF signaling in penile SCC are unclear. This study aims to investigate four key members of this pathway in penile SCC. We examined the expression of EGFR and RAS-association domain family 1 A (RASSF1A) as well as the mutation status of K-RAS and BRAF in 150 cases of penile SCC. EGFR and RASSF1A expression was evaluated by immunohistochemistry. KRAS mutations at codons 12 and 13, and the BRAF mutation at codon 600 were analyzed on DNA isolated from formalin fixed paraffin embedded tissues by direct genomic sequencing. EGFR expression was positive in all specimens, and its over-expression rate was 92%. RASSF1A expression rate was only 3.42%. Significant correlation was not found between the expression of EGFR or RASSF1A and tumor grade, pT stage or lymph node metastases. The detection of KRAS and BRAF mutations analysis was performed in 94 and 83 tumor tissues, respectively. We found KRAS mutation in only one sample and found no BRAF V600E point mutation. In summary, we found over-expression of EGFR in the majority cases of penile SCC, but only rare expression of RASSF1A, rare KRAS mutation, and no BRAF mutation in penile SCC. These data suggest that anti-EGFR agents may be potentially considered as therapeutic options in penile SCC.
- Subjects :
- Male
Gene Expression
lcsh:Medicine
medicine.disease_cause
Molecular Cell Biology
Signaling in Cellular Processes
Epidermal growth factor receptor
lcsh:Science
Skin Tumors
Aged, 80 and over
Mutation
Multidisciplinary
Signaling in Selected Disciplines
Middle Aged
ErbB Receptors
Oncology
Lymphatic Metastasis
DNA methylation
Carcinoma, Squamous Cell
Medicine
Immunohistochemistry
KRAS
Immunohistochemical Analysis
Research Article
Signal Transduction
Adult
Proto-Oncogene Proteins B-raf
Penile Neoplasm
Ras Signaling
Biology
Signaling Pathways
Young Adult
Genetic Mutation
Genetics
Cancer Genetics
Carcinoma
medicine
Humans
Squamous Cell Carcinoma
Penile Neoplasms
neoplasms
Aged
Neoplasm Staging
Oncogenic Signaling
Tumor Suppressor Proteins
Point mutation
lcsh:R
Cancers and Neoplasms
medicine.disease
digestive system diseases
stomatognathic diseases
Immunologic Techniques
ras Proteins
Cancer research
biology.protein
Clinical Immunology
lcsh:Q
Neoplasm Grading
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....643f2df8fff90b9c961411600ff54159