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Nicotinamide phosphoribosyltransferase (Nampt) may serve as the marker for osteoblast differentiation of bone marrow-derived mesenchymal stem cells
- Source :
- Experimental Cell Research. 352:45-52
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Decreased bone volume and strength with aging and enhanced risk of fractures are in part due to reduced number of bone-forming mesenchymal stem cells (MSCs) and cellular dysfunction. In a previous study, we found that osteogenic differentiation of the multipotent and omnipotent preosteoblasts are accompanied by the alterations of intracellular NAD metabolism in which nicotinamide phosphoribosyltransferase (Nampt) plays a regulatory role. The increased Nampt during osteoblast differentiation, the enzyme catalyzing NAD resynthesis from nicotinamide was noted. However, whether Nampt will also be able to affect osteogenic differentiation of primary bone marrow-derived mesenchymal stem cells (BM-MSCs), it is still uncertain. Here we report the role of Nampt in regulating osteoblast differentiation in primary mouse BM-MSCs. We found that Nampt expression was progressively elevated during BM-MSCs osteogenic differentiation. The Nampt inhibitor FK866 or knock-down of Nampt in BM-MSCs led to declined osteoblastogenesis, including attenuated ALP activity, diminished matrix mineralization and down-regulated osteoblast specific marker genes. In addition, declined osteoblastogenesis by Nampt deficiency or addition of FK866 was related to lower intracellular NAD concentration and decreased Sirt1 activity. The present findings demonstrate that osteogenic differentiation in MSCs can be modulated by intracellular NAD metabolism, in which Nampt may serve as an applicable marker for the osteoblast determination.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Blotting, Western
Nicotinamide phosphoribosyltransferase
Mice, Nude
Apoptosis
Biology
Real-Time Polymerase Chain Reaction
Mice
03 medical and health sciences
chemistry.chemical_compound
Sirtuin 1
Bone Marrow
Osteogenesis
Internal medicine
medicine
Animals
Humans
RNA, Messenger
Nicotinamide Phosphoribosyltransferase
Cells, Cultured
Cell Proliferation
Osteoblasts
Nicotinamide
Reverse Transcriptase Polymerase Chain Reaction
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Osteoblast
Cell Biology
NAD
Cell biology
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Primary bone
chemistry
Cytokines
NAD+ kinase
Bone marrow
Intracellular
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 352
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....64509933f9e37cd61206a76ac5abef1f