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Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
- Source :
- Biomolecules, Volume 10, Issue 11
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Background: The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have significant roles in allergic disease<br />however, the significance of 15-LOX-1 in CRS is not well understood. The objective of this study was to demonstrate the expression of 15-LOX-1 in CRS. Methods: The mRNA expression levels of 15-LOX-1 and periostin in nasal tissues were measured by quantitative real-time polymerase chain reaction. We also performed an immunofluorescence study of nasal tissues. Cells of the Eol-1 eosinophilic leukemic cell line were stimulated with interleukin-33 to test the induction of 15-LOX-1. Results: The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Moreover, interleukin-33 enhanced 15-LOX-1 expression in Eol-1 cells. Conclusions: 15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS.
- Subjects :
- Adult
Male
0301 basic medicine
Rhinorrhea
Gene Expression
Periostin
Immunofluorescence
Biochemistry
Article
03 medical and health sciences
0302 clinical medicine
Eosinophilic
otorhinolaryngologic diseases
medicine
Arachidonate 15-Lipoxygenase
Humans
Nasal polyps
Sinusitis
Molecular Biology
Aged
periostin
Messenger RNA
integumentary system
medicine.diagnostic_test
business.industry
15-LOX-1
Middle Aged
respiratory system
Eosinophil
medicine.disease
Interleukin 33
030104 developmental biology
medicine.anatomical_structure
030228 respiratory system
Cell culture
Chronic Disease
Immunology
IL-33
Female
eosinophils
business
Cell Adhesion Molecules
hormones, hormone substitutes, and hormone antagonists
CRS
Subjects
Details
- ISSN :
- 2218273X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Biomolecules
- Accession number :
- edsair.doi.dedup.....6489fcf9859fda5cf553bc270d953664