Back to Search
Start Over
Metaproteomics reveals metabolic transitions between healthy and diseased stony coralMussismilia braziliensis
- Source :
- Molecular Ecology. 25:4632-4644
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Infectious diseases such as white plague syndrome (WPS) and black band disease (BBD) have caused massive coral loss worldwide. We performed a metaproteomic study on the Abrolhos coral Mussismilia braziliensis to define the types of proteins expressed in healthy corals compared to WPS- and BBD-affected corals. A total of 6363 MS/MS spectra were identified as 361 different proteins. Healthy corals had a set of proteins that may be considered markers of holobiont homoeostasis, including tubulin, histone, Rab family, ribosomal, peridinin-chlorophyll a-binding protein, F0F1-type ATP synthase, alpha-iG protein, calmodulin and ADP-ribosylation factor. Cnidaria proteins found in healthy M. braziliensis were associated with Cnidaria-Symbiodinium endosymbiosis and included chaperones (hsp70, hsp90 and calreticulin), structural and membrane modelling proteins (actin) and proteins with functions related to intracellular vesicular traffic (Rab7 and ADP-ribosylation factor 1) and signal transduction (14-3-3 protein and calmodulin). WPS resulted in a clear shift in the predominance of proteins, from those related to aerobic nitrogen-fixing bacteria (i.e. Rhizobiales, Sphingomonadales and Actinomycetales) in healthy corals to those produced by facultative/anaerobic sulphate-reducing bacteria (i.e. Enterobacteriales, Alteromonadales, Clostridiales and Bacteroidetes) in WPS corals. BBD corals developed a diverse community dominated by cyanobacteria and sulphur cycle bacteria. Hsp60, hsp90 and adenosylhomocysteinase proteins were produced mainly by cyanobacteria in BBD corals, which is consistent with elevated oxidative stress in hydrogen sulphide- and cyanotoxin-rich environments. This study demonstrates the usefulness of metaproteomics for gaining better comprehension of coral metabolic status in health and disease, especially in reef systems such as the Abrolhos that are suffering from the increase in global and local threatening events.
- Subjects :
- Proteomics
0301 basic medicine
Enterobacteriales
Coral
030106 microbiology
Microbiology
03 medical and health sciences
Tandem Mass Spectrometry
Alteromonadales
Genetics
medicine
Animals
Symbiosis
Ecology, Evolution, Behavior and Systematics
Bacteria
biology
Endosymbiosis
Ecology
fungi
technology, industry, and agriculture
Bacteroidetes
Black band disease
biochemical phenomena, metabolism, and nutrition
Anthozoa
medicine.disease
biology.organism_classification
Holobiont
030104 developmental biology
Metaproteomics
Brazil
Subjects
Details
- ISSN :
- 09621083
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Molecular Ecology
- Accession number :
- edsair.doi.dedup.....648dbd96a1a092aa81f6bd090aad4750