Supplemental Figures and Reagent Table from Adaptive NK Cells with Low TIGIT Expression Are Inherently Resistant to Myeloid-Derived Suppressor Cells

Supplemental figure. 1: Gating strategy of conventional and adaptive NK cells in healthy donors and MDS patients. Supplemental figure. 2: Gating strategy for MDSC and monocytes, CD16 expression on conventional and adaptive NK cells, expression of different NK cell receptors following co-culture with monocytes or MDSC, and differential production of IFNg on TIGIT high/low NK cells. Supplemental figure. 3: Evaluation of TIGIT blocking antibodies. IFNg production following DNAM-1 and TIGIT co-blocking. NK cell TNFa production in conventional and adaptive NK cells cultured with monocytes or MDSC. Supplemental Table. 1: All antibodies used throughout the study including clone, manufactory, fluorochrome, and marker.