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The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats
- Source :
- Renal Failure. 38:294-304
- Publication Year :
- 2015
- Publisher :
- Informa UK Limited, 2015.
-
Abstract
- Diabetic nephropathy (DN) is one of the most common chronic complications of diabetes, which is associated with an increased oxidative stress induced by hyperglycemia and alterations in DJ-1/NF-E2-related factor-2 (Nrf2) pathway. In the present study, we investigated the role and the proper time nodes of DJ-1/Nrf2 pathway in the pathogenesis of DN. Diabetes mellitus (DM) model of rats was induced by intraperitoneal injection of streptozotocin (STZ) on male Sprague-Dawley (SD) rats. Then, the diabetic rats were divided into 4, 8 and 12 weeks groups. As early at 4 weeks of diabetes, renal histologic evaluation score, cystatin C (Cys C), β2-microglobulin (β2-MG) and malondialdehyde (MDA) levels were increased, and total antioxidative capacity (T-AOC) level was decreased as compared with that in the control group. The protein expressions of DJ-1, NF-E2-related factor-2 (Nrf2) and heme oxygenase-1 (HO-1) were upregulated compared with the control group from 4 weeks and further increased with the progression of DM. The protein expressions of DJ-1, Nrf2 and HO-1 in renal tissues have good line correlations with renal histologic evaluation score, respectively. Taken together, these results suggested that the activation of DJ-1/Nrf2 pathway was involved in the pathogenesis of diabetic nephropathy in rats.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
NF-E2-Related Factor 2
medicine.medical_treatment
Protein Deglycase DJ-1
Intraperitoneal injection
Critical Care and Intensive Care Medicine
medicine.disease_cause
Rats, Sprague-Dawley
Pathogenesis
Diabetic nephropathy
03 medical and health sciences
chemistry.chemical_compound
Diabetes mellitus
Internal medicine
Animals
Medicine
Diabetic Nephropathies
biology
business.industry
General Medicine
medicine.disease
Streptozotocin
Malondialdehyde
Rats
030104 developmental biology
Endocrinology
Cystatin C
chemistry
Nephrology
biology.protein
business
Oxidative stress
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 15256049 and 0886022X
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Renal Failure
- Accession number :
- edsair.doi.dedup.....64f811b653c1f82158cbc7cff8b598ce