Back to Search
Start Over
Induction of cystathionine gamma-lyase expression and metallothionein-1 S-sulfhydration alleviate cadmium-induced cell death in myoblast cells
- Source :
- Ecotoxicology and Environmental Safety. 179:222-231
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Hydrogen sulfide (H2S), a multifunctional gasotransmitter, participates in a wide range of cellular signal transduction and pathophysiological processes. Cystathionine gamma-lyase (CSE) acts as a major H2S-generating enzyme in peripheral organs and tissues. As a cysteine-rich and heavy metal-binding protein, metallothionein-1 (MT-1) is known to protect cells from various environmental stresses. Here we demonstrated that exposure of cadmium (Cd) induced oxidative stress, depleted intracellular thiols, and stimulated apoptotic cell death in mouse myoblast cells. CSE expression and H2S production were significantly enhanced by Cd treatment. NaHS, a well-known H2S donor, at physiologically relevant concentration significantly alleviated Cd-induced damage in both myoblasts and mouse skeletal muscles. In contrast, down-regulation of CSE/H2S system deteriorated Cd-stimulated oxidative stress and cell death. Exposure of the cells to Cd lead to increased expressions of metal regulatory transcription factor 1 and MT-1, while siRNA-mediated MT-1 knockdown alleviated Cd-induced CSE expression and caused more oxidative stress and cell death. In addition, H2S post-translationally modified MT-1 by S-sulfhydration and stabilized zinc-protein complex. Taken together, these data suggest that CSE/H2S system would protect myoblasts and skeletal muscles from Cd-induced damage by S-sufhydrating MT-1.
- Subjects :
- Programmed cell death
Cell Survival
Health, Toxicology and Mutagenesis
Apoptosis
Sulfides
medicine.disease_cause
Metal regulatory transcription factor 1
Cell Line
Myoblasts
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Metallothionein
Myocyte
Hydrogen Sulfide
Sulfhydryl Compounds
030304 developmental biology
0303 health sciences
biology
Chemistry
Cystathionine gamma-lyase
Cystathionine gamma-Lyase
Public Health, Environmental and Occupational Health
General Medicine
equipment and supplies
Pollution
Cystathionine beta synthase
Cell biology
Oxidative Stress
Gene Expression Regulation
030220 oncology & carcinogenesis
biology.protein
Environmental Pollutants
Intracellular
Oxidative stress
Cadmium
Subjects
Details
- ISSN :
- 01476513
- Volume :
- 179
- Database :
- OpenAIRE
- Journal :
- Ecotoxicology and Environmental Safety
- Accession number :
- edsair.doi.dedup.....652c7779a7cc96b6ee70dc392ceb1cc1