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First electrochemical immunosensor for the rapid detection of mustard seeds in plant food extracts
- Source :
- Talanta. 219
- Publication Year :
- 2020
-
Abstract
- This paper describes the first biosensor reported to date for the determination of mustard seed traces. The biosensor consists of an amperometric immunosensing platform able to sensitively and selectively determine Sin a 1 content, the major allergen of yellow mustard and the most abundant protein of these seeds. The immunosensing platform exploits the coupling of magnetic microbeads (MBs) modified with sandwich-type immune complexes, comprising polyclonal and monoclonal antibodies, selective to the target protein for its capturing and detection, respectively. In addition, a HRP-conjugated secondary antibody was used for enzymatic labelling of the monoclonal antibody, and amperometric transduction was made at screen-printed carbon electrodes (SPCEs) using the hydroquinone (HQ)/H2O2 system. The electrochemical immunosensor allows the simple and fast detection (a single 1-h incubation step) of Sin a 1 with a limit of detection of 0.82 ng mL−1 (20.5 pg of protein in 25 μL of sample) with high selectivity against structurally similar non-target allergenic proteins (such as Pin p 1 from pine nut). The developed immunoplatform was successfully used for the analysis of peanut, rapeseed, cashew, pine nut and yellow mustard extracts, giving only positive response for the yellow mustard extract with a Sin a 1 content, in full agreement with that provided by conventional ELISA methodology.
- Subjects :
- food.ingredient
medicine.drug_class
02 engineering and technology
Biosensing Techniques
Monoclonal antibody
01 natural sciences
Analytical Chemistry
food
Limit of Detection
medicine
Electrodes
Detection limit
Immunoassay
Chromatography
biology
Chemistry
Plant Extracts
010401 analytical chemistry
food and beverages
Mustard seed
Electrochemical Techniques
Hydrogen Peroxide
021001 nanoscience & nanotechnology
Primary and secondary antibodies
Amperometry
0104 chemical sciences
Polyclonal antibodies
Food
Seeds
biology.protein
Target protein
0210 nano-technology
Biosensor
Mustard Plant
Subjects
Details
- ISSN :
- 18733573
- Volume :
- 219
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....6537239c94aae448df0b2e942a05cc04