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Detection and quantification of antibody to SARS CoV 2 receptor binding domain provides enhanced sensitivity, specificity and utility
- Source :
- 2022, ' Detection and Quantification of Antibody to SARS CoV 2 Receptor Binding Domain provides enhanced Sensitivity, Specificity and Utility ', Journal of Virological Methods, vol. 302, 114475, pp. 114475 . https://doi.org/10.1016/j.jviromet.2022.114475, Journal of Virological Methods
- Publication Year :
- 2022
- Publisher :
- The Francis Crick Institute, 2022.
-
Abstract
- Accurate and sensitive detection of antibody to SARS-CoV-2 remains an essential component of the pandemic response. Measuring antibody that predicts neutralising activity and the vaccine response is an absolute requirement for laboratory-based confirmatory and reference activity.\ud \ud The viral receptor binding domain (RBD) constitutes the prime target antigen for neutralising antibody. A double antigen binding assay (DABA), providing the most sensitive format has been exploited in a novel hybrid manner employing a solid-phase S1 preferentially presenting RBD, coupled with a labelled RBD conjugate, used in a two-step sequential assay for detection and measurement of antibody to RBD (anti-RBD).\ud \ud This class and species neutral assay showed a specificity of 100 % on 825 pre COVID-19 samples and a potential sensitivity of 99.6 % on 276 recovery samples, predicting quantitatively the presence of neutralising antibody determined by pseudo-type neutralization and by plaque reduction. Anti-RBD is also measurable in ferrets immunised with ChadOx1 nCoV-19 vaccine and in humans immunised with both AstraZeneca and Pfizer vaccines. This assay detects anti-RBD at presentation with illness, demonstrates its elevation with disease severity, its sequel to asymptomatic infection and its persistence after the loss of antibody to the nucleoprotein (anti-NP). It also provides serological confirmation of prior infection and offers a secure measure for seroprevalence and studies of vaccine immunisation in human and animal populations.\ud \ud The hybrid DABA also displays the attributes necessary for the detection and quantification of anti-RBD to be used in clinical practice. An absence of detectable anti-RBD by this assay predicates the need for passive immune prophylaxis in at-risk patients.
- Subjects :
- Sars-CoV-2
Keywords: Sars-CoV-2
Infectious Disease
Receptor Binding Domain
Antibodies, Viral
Biochemistry & Proteomics
Antibodies
Ecology,Evolution & Ethology
Seroepidemiologic Studies
1108 Medical Microbiology
ChAdOx1 nCoV-19
Virology
Animals
Humans
antibodies
ISARIC4C Investigators
Chemical Biology & High Throughput
Genome Integrity & Repair
Ferrets
COVID-19
Antibodies, Neutralizing
Spike Glycoprotein, Coronavirus
RNA, Viral
Cell Cycle & Chromosomes
ELISA
Protocols
0605 Microbiology
Receptor binding domain
Structural Biology & Biophysics
Subjects
Details
- ISSN :
- 01660934
- Database :
- OpenAIRE
- Journal :
- 2022, ' Detection and Quantification of Antibody to SARS CoV 2 Receptor Binding Domain provides enhanced Sensitivity, Specificity and Utility ', Journal of Virological Methods, vol. 302, 114475, pp. 114475 . https://doi.org/10.1016/j.jviromet.2022.114475, Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....654329d716a1a0090828c9e2aa358714
- Full Text :
- https://doi.org/10.25418/crick.19114967