Subalpine dwarf shrubs differ in vulnerability to xylem cavitation: An innovative staining approach enables new insights

Dwarf shrubs are a key functional group of the high-elevation vegetation belt. Despite their ecological relevance and high sensitivity to environmental changes, the hydraulic adaptations and species-specific variations in drought tolerance within this growth form are mostly unknown. Here, we assess the xylem vulnerability to cavitation of important character species of the Alpine dwarf shrub heaths in Central Europe. Due to the high percentage of nonfunctional xylem areas in these species, vulnerability curves were gained by an innovative staining approach with safranin, validated by hydraulic and xylem anatomical measurements. The loss of 50% conducting xylem area appeared in the range of -1.78 to -2.91 MPa. Midday plant water potential during an intense summer drought remained above these critical thresholds but was correlated with cavitation resistance. No trade-off between hydraulic safety and efficiency was detected across analyzed species. We conclude that the characteristic clustered occurrence of species in the heterogeneous mountain terrain (mainly interpreted as a consequence of varying snow cover dynamics) is also reflected in species-specific adjustments in xylem hydraulics. The interspecific variety in vulnerability thresholds and plant water potentials during summer drought indicates different hydraulic strategies and adjustments in water relations between these co-occurring shrubs.