Back to Search
Start Over
Effects of Space Flight on Expression of Key Proteins in Rice Leaves
- Source :
- Rice Science, Vol 27, Iss 5, Pp 423-433 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth- and development-related physiological processes and metabolic pathways of rice different stages, the seeds of rice variety DN423 were sent into orbit for 12.5 d aboard the SJ-10 Returning Satellite, and then the seedlings of both treated and control rice were compared at the three-leaf stage (TLS) and tillering stage (TS). In addition to comparing plant growth and reactive oxygen species (ROS) levels, seedling proteomes were also compared using isobaric tags for relative and absolute quantitation (iTRAQ). Space flight increased TLS plant height by 20%, reduced and increased ROS levels of the TLS and TS seedlings, respectively, and affected the expression of 36 and 323 proteins in TLS and TS leaves, respectively. Furthermore, the functions of the differentially abundant proteins were mainly associated with metabolism, energy, and protein synthesis and degradation. These results suggested that the exposure of seeds to outer space conditions affects the subsequent abundance of key signaling proteins, gene expression, and the processes of protein synthesis and degradation, thereby affecting metabolic processes and promoting adaptation to the abiotic stress of outer space. As such, the present study sheds light on the effects of space flight on plants and contributes to a more comprehensive understanding of extraterrestrial biology.
- Subjects :
- Proteomics
0106 biological sciences
0301 basic medicine
Plant Science
lcsh:Plant culture
01 natural sciences
03 medical and health sciences
Gene expression
Protein biosynthesis
lcsh:SB1-1110
Space flight
chemistry.chemical_classification
Reactive oxygen species
biology
Abiotic stress
food and beverages
Metabolism
biology.organism_classification
Cell biology
Isobaric tags for relative and absolute quantitation
Metabolic pathway
030104 developmental biology
chemistry
Seedling
Proteome
Differentially abundant protein
Rice
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 16726308
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Rice Science
- Accession number :
- edsair.doi.dedup.....659b4d91b03de14d46ead4c0121d8dd8