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Polymorphic chloroplast microsatellite loci in Nelumbo (Nelumbonaceae)
- Source :
- American Journal of Botany. 99:e240-e244
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- PREMISE OF THE STUDY To study population genetics, phylogeography, and hybridization of Nelumbo (Nelumbonaceae), chloroplast microsatellite markers were developed. METHODS AND RESULTS Seventeen microsatellite loci were identified from the chloroplast genomes of N. nucifera and N. lutea. Polymorphisms were assessed in three populations of N. nucifera and one population of N. lutea. Nine loci were found to be polymorphic in N. nucifera, and all 17 loci were found to be polymorphic in N. lutea. In N. nucifera, the number of alleles per locus ranged from two to six, and the unbiased haploid diversity per locus ranged from 0.198 to 0.790. In N. lutea, the number of alleles ranged from two to four, and the unbiased haploid diversity per locus ranged from 0.245 to 0.694. CONCLUSIONS The identified chloroplast simple sequence repeat markers will be useful for the study of genetic diversity, phylogeography, and identification of Nelumbo cultivars.
- Subjects :
- Chloroplasts
DNA, Plant
Molecular Sequence Data
Population
Locus (genetics)
Plant Science
Nelumbo
Nelumbonaceae
Polymerase Chain Reaction
Gene Frequency
Species Specificity
Genetics
Allele
education
Alleles
Ecology, Evolution, Behavior and Systematics
Genetic diversity
education.field_of_study
Polymorphism, Genetic
biology
DNA, Chloroplast
Genetic Variation
Sequence Analysis, DNA
biology.organism_classification
Phylogeography
Microsatellite
Ploidy
Microsatellite Repeats
Subjects
Details
- ISSN :
- 15372197 and 00029122
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- American Journal of Botany
- Accession number :
- edsair.doi.dedup.....659d102cfb8c4ac97d91d69ae1943752
- Full Text :
- https://doi.org/10.3732/ajb.1100547