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Long noncoding RNA HAGLR acts as a microRNA‐143‐5p sponge to regulate epithelial‐mesenchymal transition and metastatic potential in esophageal cancer by regulating LAMP3
- Source :
- The FASEB Journal. 33:10490-10504
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Homeobox D gene cluster antisense growth-associated long noncoding RNA (HAGLR) functions as a crucial regulator in the progression and development of human cancers. We analyzed effects of HAGLR, microRNA (miR)-143-5p and lysosome-associated membrane glycoprotein (LAMP)3 on esophageal cancer (EC) and the related mechanisms. Microarray analysis was used to screen out EC-related genes and the regulation network among HAGLR, miR-143-5p, and LAMP3. The regulatory mechanisms of HAGLR and miR-143-5p in EC were analyzed following the treatment of miR-143-5p mimic, miR-143-5p inhibitor, HAGLR vector, or small interfering RNA against HAGLR in EC cells. The expression of N-cadherin, vimentin, Twist1, Snail1, and E-cadherin as well as the abilities of cell proliferation, invasion, and migration were measured. The effects of the HAGLR/miR-143-5p/LAMP3 axis were determined in vivo by assessing tumor formation in nude mice. The expression of HAGLR and LAMP3 was increased, whereas that of miR-143-5p was diminished in EC tissues and cells. HAGLR could competitively bind to miR-143-5p, and miR-143-5p targeted LAMP3. Down-regulated HAGLR or up-regulated miR-143-5p increased E-cadherin expression and significantly diminished expression of LAMP3, N-cadherin, vimentin, Twist1, and Snail1. Moreover, down-regulated HAGLR inhibited cell proliferation, invasion, migration, epithelial-mesenchymal transition (EMT), and tumor growth. Moreover, down-regulation of HAGLR inhibited LAMP3 expression by sponging miR-143-5p, thereby suppressing the progression of EC. Taken together, our results suggest HAGLR acts as a competing endogenous RNA of miR-143-5p to increase the expression of LAMP3, thus promoting EMT, proliferation, invasion, and migration in EC cells.-Yang, C., Shen, S., Zheng, X., Ye, K., Sun, Y., Lu, Y., Ge, H. Long noncoding RNA HAGLR acts as a microRNA-143-5p sponge to regulate epithelial-mesenchymal transition and metastatic potential in esophageal cancer by regulating LAMP3.
- Subjects :
- Male
0301 basic medicine
Small interfering RNA
Epithelial-Mesenchymal Transition
Esophageal Neoplasms
Mice, Nude
Apoptosis
Vimentin
Biochemistry
Mice
03 medical and health sciences
0302 clinical medicine
Cell Movement
microRNA
Tumor Cells, Cultured
Genetics
Animals
Humans
Epithelial–mesenchymal transition
Molecular Biology
Cell Proliferation
biology
Competing endogenous RNA
Cell growth
Chemistry
Microarray analysis techniques
Lysosome-Associated Membrane Glycoproteins
Middle Aged
Prognosis
Xenograft Model Antitumor Assays
Long non-coding RNA
Neoplasm Proteins
Cell biology
Gene Expression Regulation, Neoplastic
Survival Rate
MicroRNAs
030104 developmental biology
biology.protein
Female
RNA, Long Noncoding
Esophageal Squamous Cell Carcinoma
030217 neurology & neurosurgery
Biotechnology
Subjects
Details
- ISSN :
- 15306860 and 08926638
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi.dedup.....65cce49115319ae71ac3422cf072e6ad