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Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
- Source :
- Journal of Visualized Experiments.
- Publication Year :
- 2017
- Publisher :
- MyJove Corporation, 2017.
-
Abstract
- Traditional herbal medicine, an alternative medicine in the clinical setting, has received increased attention in recent years. Before delivery to the body, an additional extraction procedure is commonly required to release the active constituents from raw herbs. Water decoction is a classical extraction procedure that is still broadly used in the clinical settings. Here, we propose a detailed protocol for er-xian decoction (EXD) in order to apply herbal decoctions to experimental studies. The calculation of an animal-appropriate dose is described, as well as the four main steps of EXD: soaking, water decoction, filtration, and concentration. In addition, serum-containing EXD is introduced to rats as a means of in vitro validation. Here, rats were orally administered EXD for three days. Blood samples were then collected, inactivated, centrifuged, and filtered. The serum, diluted with the culture medium, can be utilized to treat cells or tissues in vitro. For example, EXD was applied to both in vivo and in vitro studies and demonstrated that EXD enhances osteogenesis. This protocol can be used as a reference for the preparation and application of herbal medicines.
- Subjects :
- 0301 basic medicine
General Immunology and Microbiology
Traditional medicine
business.industry
General Chemical Engineering
General Neuroscience
Clinical settings
Decoction
Traditional Chinese medicine
Pharmacology
General Biochemistry, Genetics and Molecular Biology
In vitro
law.invention
03 medical and health sciences
030104 developmental biology
In vivo
law
Medicine
Serum pharmacology
business
Phytotherapy
Er xian decoction
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....65d8e21f27cb9be7db347ba3eadcfbbe