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Saturated Reconstruction of a Volume of Neocortex
- Source :
- Cell. (3):648-661
- Publisher :
- Elsevier Inc.
-
Abstract
- SummaryWe describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which all cellular objects (axons, dendrites, and glia) and many sub-cellular components (synapses, synaptic vesicles, spines, spine apparati, postsynaptic densities, and mitochondria) are rendered and itemized in a database. We explore these data to study physical properties of brain tissue. For example, by tracing the trajectories of all excitatory axons and noting their juxtapositions, both synaptic and non-synaptic, with every dendritic spine we refute the idea that physical proximity is sufficient to predict synaptic connectivity (the so-called Peters’ rule). This online minable database provides general access to the intrinsic complexity of the neocortex and enables further data-driven inquiries.Video Abstract
- Subjects :
- Neurons
Neocortex
Dendritic spine
Biochemistry, Genetics and Molecular Biology(all)
Dendrites
Microtomy
Biology
Synaptic vesicle
Spinal column
Axons
General Biochemistry, Genetics and Molecular Biology
Synapse
Automation
Mice
medicine.anatomical_structure
Cerebral cortex
Postsynaptic potential
Synapses
Microscopy, Electron, Scanning
medicine
Excitatory postsynaptic potential
Animals
Synaptic Vesicles
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 00928674
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....660a2a62cff47fce204afca98a899b7c
- Full Text :
- https://doi.org/10.1016/j.cell.2015.06.054