Additional file 2 of CircGNB1 facilitates the malignant phenotype of GSCs by regulating miR-515-5p/miR-582-3p-XPR1 axis

Additional file 2. Fig. S2: CircGNB1 promoted the malignant phenotype of GSCs via IL6/JAK2/STAT3 pathway. a QRT-PCR showed that anti-IL6 treatment could reverse the expression of IL6 promoted by circGNB1 overexpression. b ELISA assays showed that circGNB1 was positively related to the IL6 expression. c Western blotting assays showed that circGNB1 overexpression promoted IL6/JAK2/STAT3 expression, while circGNB1 knockdown inhibited the pathway. d–g The edu (d, e) and transwell (f, g) assays revealed that anti-IL6 treatment could reverse the promoting effect caused by circGNB1 overexpression. h–j The neurosphere formation assays showed that circGNB1 overexpression promoted the neurosphere formation, while anti-IL6 treatment could inhibited the size of neurosphere.