In vitro evaluation of the cyto- and genotoxicity of titanium and titanium-based alloy Ti-6Al-4V used in the manufacture of screw type dental implants - preliminary study

Introduction. Titanium and the alloys that contain this element became in the last decade of the twentieth century almost irreplaceable materials in many biomedical fields, including dentistry. Purpose. The purpose of this study is to test and evaluate the cyto- and genotoxicity of Ti4 and Ti-6Al-4V, to obtain biomaterials with antimicrobial properties, compatible with the human body. Material and method. Ti4 and Ti-4Al-6V in powder form were analyzed using cell proliferation analysis and micronucleus test. Two concentrations of 1.5 mg/ml and 3 mg/ml were tested for each type of sample. Results and discussions. For both materials tested in the two concentrations, the presence of micronuclei was observed, but within normal limits. Conclusions. Although the variations are within normal limits, it should be noted that for Ti-6Al-4V the incidence was higher at both concentrations than in the case of Ti4.