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Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains
- Source :
- ACS Synthetic Biology. 6:211-216
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Luminescence
Biomedical Engineering
Biology
Sensitivity and Specificity
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Genome
Paired design
03 medical and health sciences
Nucleic Acids
Luciferase
Luciferases
Sequence (medicine)
Genetics
Base Sequence
Colocalization
Mycobacterium tuberculosis
General Medicine
Endonucleases
Dna detection
030104 developmental biology
Nucleic acid
Mycobacterium tuberculosis DNA
CRISPR-Cas Systems
Genome, Bacterial
Subjects
Details
- ISSN :
- 21615063
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- ACS Synthetic Biology
- Accession number :
- edsair.doi.dedup.....66cb002ca31f277029a80e8fffdeb2a3