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BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes
- Source :
- Journal of Biomedicine and Biotechnology, Journal of Biomedicine and Biotechnology, Vol 2011 (2011)
- Publication Year :
- 2011
- Publisher :
- Hindawi Limited, 2011.
-
Abstract
- Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance geneDnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistance geneGb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective.
- Subjects :
- Chromosomes, Artificial, Bacterial
Article Subject
Library
Positional cloning
lcsh:Biotechnology
Health, Toxicology and Mutagenesis
lcsh:Medicine
Genes, Plant
Polymerase Chain Reaction
Chromosomes, Plant
Fluorescence
law.invention
law
lcsh:TP248.13-248.65
Genetics
Animals
Cloning, Molecular
Molecular Biology
Genome size
Gene
Triticum
Polymerase chain reaction
Plant Diseases
Cloning
biology
lcsh:R
Nucleic Acid Hybridization
food and beverages
Chromosome
General Medicine
biology.organism_classification
Immunity, Innate
Aphids
Karyotyping
Molecular Medicine
Russian wheat aphid
Microsatellite Repeats
Research Article
Biotechnology
Subjects
Details
- ISSN :
- 11107251 and 11107243
- Volume :
- 2011
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedicine and Biotechnology
- Accession number :
- edsair.doi.dedup.....66cb1a5adcdf33bbc7881d874d58b2bb