Additional file 4: of A functional SNP associated with atopic dermatitis controls cell type-specific methylation of the VSTM1 gene locus

Gating strategy for the flow cytometry analysis of SIRL-1 expression on whole blood samples. Whole blood samples stained with antibodies specific for CCR3, CD16, CD123, CD14, FcÎľRIÎą, and HLA-DR were counterstained with either anti-SIRL-1 or isotype-matched control antibody. Gates allowing the simultaneous analysis of the SIRL-1 staining on neutrophils, eosinophils, basophils, monocytes, mDC, pDC, B cells, and NK & T cells are indicated. (PDF 210Â kb)