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African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs
- Source :
- Virol Sin
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), an often lethal disease in domestic and wild pigs. ASF represents a major threat to the swine industry worldwide. Currently, no commercial vaccine is available because of the complexity of ASFV or biosecurity concerns. Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge. In the present study, a mutant ASFV strain with the deletion of ASFV MGF-110-9L (ASFV-Δ9L) was generated from a highly virulent ASFV CN/GS/2018 parental strain, a genotype II ASFV. Relative to the parental ASFV isolate, deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-Δ9L to replicate in vitro in primary swine macrophage cell cultures. The majority of animals inoculated intramuscularly with a low dose of ASFV-Δ9L (10 HAD(50)) remained clinically normal during the 21-day observational period. Three of five ASFV-Δ9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response, indicating partial attenuation of the ASFV-Δ9L strain in pigs. The findings imply the potential usefulness of the ASFV-Δ9L strain for further development of ASF control measures.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Swine
030106 microbiology
Immunology
Mutant
Virulence
Viremia
African swine fever virus
Viral Proteins
03 medical and health sciences
Medical microbiology
Virology
Genotype
medicine
Animals
Viral shedding
African Swine Fever
biology
Viral Vaccines
biology.organism_classification
medicine.disease
African Swine Fever Virus
Titer
030104 developmental biology
Molecular Medicine
Research Article
Subjects
Details
- ISSN :
- 1995820X and 16740769
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Virologica Sinica
- Accession number :
- edsair.doi.dedup.....671fe1c4f01d54b0480aa2104cb9cbeb