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Biological properties of pseudorecombinant and recombinant strains created with cucumber mosaic virus and tomato aspermy virus
- Source :
- Journal of Virology, Journal of Virology, American Society for Microbiology, 1997, 71 (5), pp.3597-3602. ⟨10.1128/JVI.71.5.3597-3602.1997⟩
- Publication Year :
- 1997
- Publisher :
- American Society for Microbiology, 1997.
-
Abstract
- International audience; Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) are closely related cucumoviruses, We have made pseudorecombinant viruses in which the RNAs 3 of these two viruses have been exchanged and recombinant viruses containing chimeric RNA 3 molecules, in which the coat proteins and the 3'-end regions of CMV and TAV have been exchanged, giving rise to recombinants designated RT3 and TR3, The replication properties and the cell-to-cell and long-distance movement patterns of these pseudorecombinant and recombinant viruses were examined in different hosts, All the viruses were able to replicate and accumulate RNA 4 in protoplasts, The pseudorecombinants and the R1R2RT3 recombinant infected tobacco systemically, but the R1R2TR3 recombinant was not detectable, even in the inoculated leaves, Comparison of the abilities of the viruses to replicate in protoplasts and intact cucumber plants suggests that cell-to cell movement factors are also encoded by RNAs 1 and/or 2, Major determinants of symptom severity in Nicotiana glutinosa are localized on the 3' part of RNA 3, and in Nicotiana benthamiana, more severe symptoms were observed with the T1T2R3 strain than,vith the others tested.
- Subjects :
- 0106 biological sciences
viruses
Immunology
Nicotiana benthamiana
Biology
Cucumovirus
01 natural sciences
Microbiology
law.invention
Cucumber mosaic virus
03 medical and health sciences
Solanum lycopersicum
law
Virology
Plant virus
Tomato aspermy virus
Nicotiana glutinosa
030304 developmental biology
0303 health sciences
fungi
food and beverages
RNA
biology.organism_classification
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
3. Good health
Insect Science
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Recombinant DNA
Cucumis sativus
Research Article
010606 plant biology & botany
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....674a1e355a7733fc80867ab7b1eb1b1e