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Human fusion proteins between interleukin 2 and IgM heavy chain are cytotoxic for cells expressing the interleukin 2 receptor
- Publication Year :
- 1992
-
Abstract
- We have constructed a hybrid cDNA coding for a fusion protein between human interleukin 2 and a truncated heavy chain from human immunoglobulin M. The protein encoded by this cDNA contains the entire interleukin 2 sequence including its signal peptide, fused at its C terminus to domains 2 to 4 of the immunoglobulin heavy-chain constant region. Cells transfected with the hybrid cDNA secrete multimeric forms of the fusion protein, which bind specifically to cells bearing high-affinity interleukin 2 receptors. This binding leads either to T-cell proliferation or, if complement is added, to T-cell death. Multimeric forms of the fusion protein with a molecular mass above 500 kDa mediate complement-dependent lysis but trigger proliferation inefficiently when compared with forms with a low molecular mass (< 500 kDa). In contrast, the latter efficiently mediate T-cell proliferation without inducing complement-dependent lysis. The high molecular mass fusion proteins could thus constitute valuable tools for specific immunosuppression in humans.
- Subjects :
- Cytotoxicity, Immunologic
Recombinant Fusion Proteins
Molecular Sequence Data
Interleukin 1 receptor, type II
Biology
In Vitro Techniques
Lymphocyte Activation
Polymerase Chain Reaction
Interleukin 20
Interleukin 26
Tumor Cells, Cultured
Humans
Interleukin 12 receptor, beta 1 subunit
Interleukin 3
Multidisciplinary
Base Sequence
Immunoglobulin mu-Chains
Receptors, Interleukin-2
Complement System Proteins
DNA
Molecular biology
Fusion protein
Oligodeoxyribonucleotides
Interleukin 19
Interleukin-2
Interleukin 1 receptor, type I
Cell Division
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....675a1531b7c0a6c4a695bc65060f7d13