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Membrane transfer from oocyte to sperm occurs in two CD9-independent ways that do not supply the fertilising ability of Cd9-deleted oocytes
- Source :
- Reproduction (Cambridge, England). 144(1)
- Publication Year :
- 2012
-
Abstract
- Spermatozoa undergo regulation of their functions along their lifespan through exchanges via vesicles or interactions with epithelial cells, in the epididymis, in the seminal fluid and in the female genital tract. Two different ways of oocyte membrane transfer to spermatozoa have been described: trogocytosis and exosomes. We here report an analysis ofin vitroexchanges between the membranes of unfertilised oocytes and capacitated spermatozoa. We showed that optimum conditions are fulfilled when unfertilised oocytes interact with acrosome-reacted spermatozoa, a scenario mimicking the events occurring when the fertilising spermatozoon is inside the perivitelline space. Although CD9 tetraspanin is an essential molecule for fertilisation, exosome and trogocytosis transfer persists inCd9-null oocytes in spite of their dramatic fusion failure. These exchanges are CD9 tetraspanin independent. We also confirm that mice sperm express CD9 tetraspanin and that when Cd9-null oocytes were inseminated with sperm covered with oocyte membrane materials, including CD9 tetraspanin, no rescue of the oocytes' fertilisability could be obtained. Thus, the existence of two ways of exchange between gametes during fertilisation suggests that these events could be of a physiological importance in this process.
- Subjects :
- Male
Embryology
Trogocytosis
Tetraspanins
Perivitelline space
Biology
Tetraspanin 29
Mice
Endocrinology
Tetraspanin
medicine
Animals
Fertilisation
Sperm-Ovum Interactions
Spermatozoon
urogenital system
Acrosome Reaction
Cell Membrane
Obstetrics and Gynecology
Cell Biology
Epididymis
Oocyte
Sperm
Spermatozoa
Cell biology
Microscopy, Electron
medicine.anatomical_structure
Reproductive Medicine
Fertilization
embryonic structures
Oocytes
Female
Sperm Capacitation
Subjects
Details
- ISSN :
- 17417899
- Volume :
- 144
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Reproduction (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....677689e8716ef52e554c13ebad3a2667