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TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei
- Source :
- Faktorová, D, Bär, A, Hashimi, H, McKenney, K, Horák, A, Schnaufer, A, Rubio, M A T, Alfonzo, J D & Lukeš, J 2018, ' TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei ', Molecular and Biochemical Parasitology, vol. 225, pp. 84-93 . https://doi.org/10.1016/j.molbiopara.2018.09.003
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our knowledge of this process by identifying and characterizing the essential protein TbUTP10, a homolog of yeast U3 small nucleolar RNA-associated protein 10 - UTP10 (HEATR1 in human), in the excavate parasitic protist Trypanosoma brucei. We show that TbUTP10 localizes to the nucleolus and that its ablation by RNAi knock-down in two different T. brucei life cycle stages results in similar phenotypes: a disruption of pre-18S rRNA processing, exemplified by the accumulation of rRNA precursors, a reduction of mature 18S rRNA, and also a decrease in the level of U3 snoRNA. Moreover, polysome profiles of the RNAi-induced knock-down cells show a complete disappearance of the 40S ribosomal subunit, and a prominent accumulation of the 60S large ribosomal subunit, reflecting impaired ribosome assembly. Thus, TbUTP10 is an important protein in the processing of 18S rRNA.
- Subjects :
- 0301 basic medicine
Trypanosoma brucei brucei/enzymology
Nucleolus
Trypanosoma brucei brucei
Protozoan Proteins
Biology
Ribosome
Article
Ribosome assembly
03 medical and health sciences
0302 clinical medicine
Large ribosomal subunit
RNA, Ribosomal, 18S
RNA, Small Nucleolar
Eukaryotic Small Ribosomal Subunit
Gene Silencing
RNA Processing, Post-Transcriptional
RRNA processing
Molecular Biology
RNA, Small Nucleolar/metabolism
Genes, Essential
Eukaryotic Large Ribosomal Subunit
RNA, Ribosomal, 18S/metabolism
RNA-Binding Proteins
Ribosomal RNA
RNA-Binding Proteins/genetics
Cell biology
030104 developmental biology
Protozoan Proteins/genetics
Parasitology
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 01666851
- Volume :
- 225
- Database :
- OpenAIRE
- Journal :
- Molecular and Biochemical Parasitology
- Accession number :
- edsair.doi.dedup.....678723c204ca7797fa530b10c605956a