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Microfluidic Quantitative PCR for Simultaneous Quantification of Multiple Viruses in Environmental Water Samples
- Publication Year :
- 2014
- Publisher :
- American Society for Microbiology, 2014.
-
Abstract
- To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sapovirus, and hepatitis A and E viruses. Murine norovirus and mengovirus were also quantified in our MFQPCR system as a sample processing control and an internal amplification control, respectively. River water contaminated with effluents from a wastewater treatment plant in Sapporo, Japan, was collected and used to validate our MFQPCR system for multiple viruses. High-throughput quantitative information was obtained with a quantification limit of 2 copies/μl of cDNA/DNA. Using this MFQPCR system, we could simultaneously quantify multiple viral pathogens in environmental water samples. The viral quantities obtained using MFQPCR were similar to those determined by conventional quantitative PCR. Thus, the MFQPCR system developed in this study can provide direct and quantitative information for viral pathogens, which is essential for risk assessments.
- Subjects :
- viruses
ved/biology.organism_classification_rank.species
Microfluidics
Molecular Sequence Data
Wastewater
medicine.disease_cause
Applied Microbiology and Biotechnology
Polymerase Chain Reaction
Astrovirus
law.invention
Microbiology
Japan
Rivers
law
Rotavirus
medicine
Polymerase chain reaction
Phylogeny
Ecology
biology
ved/biology
Public and Environmental Health Microbiology
Sapovirus
biology.organism_classification
Virology
Viruses
Norovirus
Enterovirus
Aichi virus
Food Science
Biotechnology
Murine norovirus
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....679a2d0118e74f796ac2e2a15806f124