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Normal mouse intestinal epithelial cells as a model for the in vitro invasion of Trichinella spiralis infective larvae
- Source :
- PLoS ONE, PLoS ONE, Vol 6, Iss 10, p e27010 (2011)
- Publication Year :
- 2011
-
Abstract
- It has been known for many years that Trichinella spiralis initiates infection by penetrating the columnar epithelium of the small intestine; however, the mechanisms used by the parasite in the establishment of its intramulticellular niche in the intestine are unknown. Although the previous observations indicated that invasion also occurs in vitro when the infective larvae are inoculated onto cultures of intestinal epithelial cells (e.g., human colonic carcinoma cell line Caco-2, HCT-8), a normal readily manipulated in vitro model has not been established because of difficulties in the culture of primary intestinal epithelial cells (IECs). In this study, we described a normal intestinal epithelial model in which T. spiralis infective larvae were shown to invade the monolayers of normal mouse IECs in vitro. The IECs derived from intestinal crypts of fetal mouse small intestine had the ability to proliferate continuously and express specific cytokeratins as well as intestinal functional cell markers. Furthermore, they were susceptible to invasion by T. spiralis. When inoculated onto the IEC monolayer, infective larvae penetrated cells and migrated through them, leaving trails of damaged cells heavily loaded with T. spiralis larval excretory-secretory (ES) antigens which were recognized by rabbit immune sera on immunofluorescence test. The normal intestinal epithelial model of invasion mimicking the natural environment in vivo will help us to further investigate the process as well as the mechanisms by which T. spiralis establishes its intestinal niche.
- Subjects :
- Pathology
Mouse
lcsh:Medicine
Fluorescent Antibody Technique
Cell Separation
Stem cell marker
Mice
Molecular Cell Biology
Microscopy, Phase-Contrast
lcsh:Science
Cells, Cultured
Mice, Inbred BALB C
Multidisciplinary
medicine.diagnostic_test
Trichinellosis
Animal Models
Intestines
medicine.anatomical_structure
Infectious Diseases
Larva
Medicine
Rabbits
Cellular Types
Research Article
medicine.medical_specialty
Trichinella spiralis
Biology
Immunofluorescence
digestive system
Models, Biological
Microbiology
Model Organisms
Antigen
Virology
medicine
Parasitic Diseases
Animals
Humans
Cell Proliferation
Staining and Labeling
lcsh:R
Epithelial Cells
biology.organism_classification
In vitro
Epithelium
Small intestine
Animal Models of Infection
Cell culture
lcsh:Q
Infectious Disease Modeling
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....679eecc5f0c7103c23f6955bda5e071c