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The 19 S Proteasome Subcomplex Establishes a Specific Protein Interaction Network at the Promoter for Stimulated Transcriptional Initiation in Vivo
- Source :
- Journal of Biological Chemistry. 284:35714-35724
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- The 26 S proteasome complex that comprises the 20 S core and 19 S regulatory (with six ATPases) particles is engaged in an ATP-dependent degradation of a variety of key regulatory proteins and, thus, controls important cellular processes. Interestingly, several recent studies have implicated the 19 S regulatory particle in controlling eukaryotic transcriptional initiation or activation independently of the 20 S core particle. However, the mechanism of action of the 19 S proteasome subcomplex in regulation of eukaryotic transcriptional activation is not clearly understood in vivo. Here, using a chromatin immunoprecipitation assay in conjunction with mutational and transcriptional analyses in Saccharomyces cerevisiae, we show that the 19 S proteasomal subcomplex establishes a specific protein interaction network at the upstream activating sequence of the promoter. Such an interaction network is essential for formation of the preinitiation complex at the core promoter to initiate transcription. Furthermore, we demonstrate that the formation of the transcription complex assembly at the promoter is dependent on 19 S ATPase activity. Intriguingly, 19 S ATPases appear to cross-talk for stimulation of the assembly of transcription factors at the promoter. Together, these results provide significant insights as to how the 19 S proteasome subcomplex regulates the formation of the active transcription complex assembly (and, hence, transcriptional initiation) at the promoter in vivo.
- Subjects :
- Proteasome Endopeptidase Complex
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Promoter
Saccharomyces cerevisiae
Cell Biology
Proteasome complex
Biology
Biochemistry
Molecular biology
Cell biology
Upstream activating sequence
Proteasome
Transcription (biology)
Mutation
Transcription preinitiation complex
Transcription, Chromatin, and Epigenetics
Promoter Regions, Genetic
Molecular Biology
Transcription factor
Chromatin immunoprecipitation
Transcription Factors
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 284
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....67b40186d3c3cccd15ffebc6eec8744a