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Characterisation of the secretome of the clam parasite, QPX
- Source :
- International journal for parasitology. 45(2-3)
- Publication Year :
- 2014
-
Abstract
- Secreted and cell surface-associated molecules play a major role in disease development processes and host-pathogen interactions, and usually determine the virulence of invading organisms. In this study, we investigated proteins secreted by quahog parasite unknown, a thraustochytrid protist that infects the hard clam, Mercenaria mercenaria. In silico analysis of quahog parasite unknown transcripts predicted over 1200 proteins to possess an amino-terminal signal peptide which directs proteins into the classical eukaryotic secretory pathway. Proteomic analysis using LC/MS technology identified 56 proteins present in the extracellular secretion of quahog parasite unknown cells grown in vitro, including six mucin-like molecules, four glycosyl hydrolases and eight peptidases. Transcription levels of 19 quahog parasite unknown extracellular proteins were investigated in clam tissue lesions (in vivo) using quantitative PCR. The overexpression of six of these extracellular proteins in clam tissues compared with in vitro cultures suggests that they are involved in interaction with the clam host.
- Subjects :
- Signal peptide
Proteomics
animal structures
Proteome
Virulence
Real-Time Polymerase Chain Reaction
Mass Spectrometry
Microbiology
Mercenaria
Extracellular
Parasite hosting
Animals
Secretion
Parasites
RNA, Messenger
Secretory pathway
biology
Gene Expression Profiling
Computational Biology
Proteins
biology.organism_classification
Infectious Diseases
Biochemistry
Parasitology
Hard clam
Chromatography, Liquid
Subjects
Details
- ISSN :
- 18790135
- Volume :
- 45
- Issue :
- 2-3
- Database :
- OpenAIRE
- Journal :
- International journal for parasitology
- Accession number :
- edsair.doi.dedup.....67d00ac30a8fea444928c89941d41c31