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Parechovirus a Detection by a Comprehensive Approach in a Clinical Laboratory
- Source :
- Viruses, Vol 10, Iss 12, p 711 (2018), Viruses, Volume 10, Issue 12
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Parechovirus A (Human parechovirus, HPeV) causes symptoms ranging from severe neonatal infection to mild gastrointestinal and respiratory disease. Use of molecular approaches with RT-PCR and genotyping has improved the detection rate of HPeV. Conventional methods, such as viral culture and immunofluorescence assay, together with molecular methods facilitate comprehensive viral diagnosis. To establish the HPeV immunofluorescence assay, an antibody against HPeV capsid protein VP0 was generated by using antigenic epitope prediction data. The specificity of the anti-HPeV VP0 antibody was demonstrated on immunofluorescence assay, showing that this antibody was specific for HPeV but not enteroviruses. A total of 74 HPeV isolates, 7 non–polio-enteroviruses and 12 HPeV negative cell culture supernatant were used for evaluating the efficiency of the anti-HPeV VP0 antibody. The sensitivity of HPeV detection by the anti-HPeV VP0 antibody was consistent with 5′untranslated region (UTR) RT-PCR analysis. This study established comprehensive methods for HPeV detection that include viral culture and observation of cytopathic effect, immunofluorescence assay, RT-PCR and genotyping. The methods were incorporated into our routine clinical practice for viral diagnosis. In conclusion, this study established a protocol for enterovirus and HPeV virus identification that combines conventional and molecular methods and would be beneficial for HPeV diagnosis.
- Subjects :
- emerging virus
0301 basic medicine
Genotyping Techniques
030106 microbiology
lcsh:QR1-502
Parechovirus
human parechovirus
Antibodies, Viral
Real-Time Polymerase Chain Reaction
Immunofluorescence
medicine.disease_cause
Article
lcsh:Microbiology
Epitope
03 medical and health sciences
Antibody Specificity
Virology
clinical laboratory
Humans
Medicine
immunofluorescence assay
Genotyping
Picornaviridae Infections
medicine.diagnostic_test
biology
Clinical Laboratory Techniques
business.industry
Viral culture
Human parechovirus
biology.organism_classification
virus diagnosis
Infectious Diseases
Fluorescent Antibody Technique, Direct
biology.protein
RNA, Viral
Enterovirus
Capsid Proteins
Antibody
business
Subjects
Details
- ISSN :
- 19994915
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....67f30c515758f7ac426522db0ea70d3c
- Full Text :
- https://doi.org/10.3390/v10120711