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The inhibitory effect of sodium nitroprusside on HIF-1 activation is not dependent on nitric oxide-soluble guanylyl cyclase pathway
- Source :
- Biochemical and Biophysical Research Communications. 324:417-423
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Adaptation to hypoxia and maintenance of O(2) homeostasis involve a wide range of responses that occur at different organizational levels in the body. One of the most important transcription factors that activate the expression of O(2)-regulated genes is hypoxia-inducible factor 1 (HIF-1). Nitric oxide (NO) mediates a variety of biological effects including relaxation of blood vessels and cytotoxicity of activated macrophages. We investigated the effect of the clinically used nitrates nitroglycerin (NTG), isosorbide dinitrate (ISDN), and sodium nitroprusside (SNP) on HIF-1-mediated transcriptional responses to hypoxia. We demonstrate that among the three nitrates, only SNP inhibits HIF-1 activation in response to hypoxia. In contrast, NTG or ISDN does not affect HIF-1 activity. SNP inhibits the accumulation of HIF-1alpha, the regulatory subunit of HIF-1, and the transcriptional activation of HIF-1alpha via a mechanism that is not dependent on either NO or soluble guanylate cyclase.
- Subjects :
- Nitroprusside
medicine.medical_specialty
GUCY1B3
Transcription, Genetic
Vasodilator Agents
Biophysics
Receptors, Cytoplasmic and Nuclear
Isosorbide Dinitrate
Pharmacology
Nitric Oxide
Biochemistry
Cell Line
Nitric oxide
Cyclic N-Oxides
Nitroglycerin
chemistry.chemical_compound
Soluble Guanylyl Cyclase
Internal medicine
medicine
Animals
Humans
Nitric Oxide Donors
Molecular Biology
G alpha subunit
GUCY1A3
Imidazoles
Nuclear Proteins
Free Radical Scavengers
Cell Biology
Guanylate cyclase 2C
Hypoxia (medical)
Hypoxia-Inducible Factor 1, alpha Subunit
DNA-Binding Proteins
Oxygen
Endocrinology
Gene Expression Regulation
chemistry
Guanylate Cyclase
Hypoxia-Inducible Factor 1
Sodium nitroprusside
medicine.symptom
Soluble guanylyl cyclase
Signal Transduction
Transcription Factors
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 324
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....6806b0e79a80c271b990990624b3155a