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Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticus
- Source :
- Journal of Agricultural and Food Chemistry. 63:7849-7854
- Publication Year :
- 2015
- Publisher :
- American Chemical Society (ACS), 2015.
-
Abstract
- A simple colorimetric aptasensor system has been developed to detect Vibrio parahemolyticus. Magnetic nanoparticles (MNPs) are synthesized and conjugated with specific aptamers against target and used as capture probes. In addition, this method employs gold nanoparticles (AuNPs) as carriers of horseradish peroxidase (HRP) and aptamers, which served as signal probes. In the presence of target, a "sandwich-type" complex of AuNPs-HRP-aptamer-target-aptamer-MNPs is formed through specific recognition of aptamers and corresponding target. As a result, HRP molecules confined at the surface of the "sandwich" complexes catalyze the enzyme substrate, 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2 and generate an optical signal. Under optimal conditions, the signals are linearly dependent on V. parahemolyticus concentrations from 10 to 10(6) colony-forming units (cfu)/mL in a logarithmic plot, with a limit of detection of 10 cfu/mL. Owing to AuNPs, a large amount of HRP could be loaded, resulting in an amplified signal, and the sensitivity would be improved. This strategy has the potential of being extended to the construction of simple monitor systems for a variety of biomolecules related to food safety.
- Subjects :
- chemistry.chemical_classification
Detection limit
biology
Benzidines
Biomolecule
Aptamer
Analytical chemistry
Metal Nanoparticles
Substrate (chemistry)
Biosensing Techniques
Hydrogen Peroxide
General Chemistry
Horseradish peroxidase
Combinatorial chemistry
chemistry
Limit of Detection
Colloidal gold
biology.protein
Magnetic nanoparticles
Colorimetry
Gold
Vibrio parahaemolyticus
General Agricultural and Biological Sciences
Horseradish Peroxidase
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....6817a03cf6ab696a3b14d1ee46dbc702