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Small DNA circles as bacterial topoisomerase I inhibitors
- Source :
- RSC Advances. 9:18415-18419
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- Bacterial topoisomerase I is a potential target during the course of antibacterial drug therapy. In our studies, specifically designed small DNA circles with high bending stress were synthesized. It is demonstrated that small DNA circles showed high inhibitory effect on the activity of bacterial topoisomerase I and the single-stranded regions associated with bending deformation in DNA circles are believed to be the crucial factor for trapping the enzymes and decreasing the effective concentration of the topoisomerases in the reaction solution. In addition, the DNA circles showed high thermal stability and excellent nuclease resistance. In consideration of the low cytotoxicity of DNA-based biopharmaceuticals, our results may provide a new idea for the future design and optimization of DNA-based therapeutic agents for antibacterial therapy.
- Subjects :
- chemistry.chemical_classification
Nuclease
biology
General Chemical Engineering
Topoisomerase
02 engineering and technology
General Chemistry
Topoisomerase-I Inhibitor
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
0104 chemical sciences
chemistry.chemical_compound
Enzyme
chemistry
Biochemistry
biology.protein
0210 nano-technology
Cytotoxicity
Antibacterial drug
Inhibitory effect
DNA
Subjects
Details
- ISSN :
- 20462069
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- RSC Advances
- Accession number :
- edsair.doi.dedup.....682a8381c2b4490684a38c7c930fed4d