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Identification of the unwinding region in the clostridioides difficile chromosomal origin of replication
- Source :
- Frontiers in Microbiology, 11. FRONTIERS MEDIA SA, Frontiers in Microbiology, Frontiers in Microbiology, Vol 11 (2020)
- Publication Year :
- 2020
-
Abstract
- Faithful DNA replication is crucial for viability of cells across all kingdoms. Targeting DNA replication is a viable strategy for inhibition of bacterial pathogens. Clostridioides difficile is an important enteropathogen that causes potentially fatal intestinal inflammation. Knowledge about DNA replication in this organism is limited and no data is available on the very first steps of DNA replication. Here, we use a combination of in silico predictions and in vitro experiments to demonstrate that C. difficile employs a bipartite origin of replication that shows DnaA-dependent melting at oriC2, located in the dnaA-dnaN intergenic region. Analysis of putative origins of replication in different clostridia suggests that the main features of the origin architecture are conserved. This study is the first to characterize aspects of the origin region of C. difficile and contributes to our understanding of the initiation of DNA replication in clostridia.
- Subjects :
- Microbiology (medical)
DnaA
P1 nuclease
In silico
lcsh:QR1-502
Origin of replication
Microbiology
lcsh:Microbiology
Clostridia
03 medical and health sciences
Intergenic region
unwinding
ddc:570
Organism
030304 developmental biology
Original Research
Genetics
oriC
0303 health sciences
biology
030306 microbiology
Clostridioides difficile
DNA replication
biology.organism_classification
In vitro
bacteria
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology, 11. FRONTIERS MEDIA SA, Frontiers in Microbiology, Frontiers in Microbiology, Vol 11 (2020)
- Accession number :
- edsair.doi.dedup.....682d4fdf054c58d3f39f1984ba3d0d5b