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Successful Enrichment of Rarely Found Candidatus Anammoxoglobus propionicus from Leachate Sludge

Authors :
Suen Shin Wong
Pun Jen Cheng
Shu Chuan Hsu
Ping Heng Hsieh
Yen Chun Lai
Chun-Hsiung Hung
Source :
Journal of Microbiology and Biotechnology. 24:879-887
Publication Year :
2014
Publisher :
Journal of Microbiology and Biotechnology, 2014.

Abstract

Bacteria that mediate the anaerobic oxidation of ammonium (anammox) have been detected in natural ecosystems, as well as various wastewater treatment systems. In this study, sludge from a particular landfill leachate anaerobic treatment system was selected as the incubation seed for anammox microorganism enrichment owing to its possible anammox activity. Transmission electron microscopy observation, denaturing gradient gel electrophoresis analysis, and cloning/sequencing techniques were applied to identify the diversity of anammox microorganisms throughout the incubation. During the early stage of operation, the diversity of anammox microorganisms was similar to the original complex microbes in the seed sludge. However, as incubation time increased, the anammox microorganism diversity within the system that was originally dominated by Candidatus (Ca.) Brocadia sp. was replaced by Ca. Anammoxoglobus propionicus. The domination of Ca. Anammoxoglobus propionicus produced a stable removal of ammonia (70 mg-N/l) and nitrite (90 mg-N/l), and the total nitrogen removal efficiency was maintained at nearly 95%. The fluorescence in situ hybridization results showed that Ca. Anammoxoglobus propionicus was successfully enriched from 1.8 ± 0.6% initially to 65 ± 5% after 481 days of operation. Therefore, the present results demonstrated the feasibility of enriching Ca. Anammoxoglobus propionicus from leachate sludge, even though the original cell count was extremely low. Application of this seldom found anammox organism could offer an alternative to current ammonia-nitrogen treatment.

Details

ISSN :
17388872 and 10177825
Volume :
24
Database :
OpenAIRE
Journal :
Journal of Microbiology and Biotechnology
Accession number :
edsair.doi.dedup.....6831fe65102a73afbd2194ce41226d9c
Full Text :
https://doi.org/10.4014/jmb.1401.01016