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Successful Enrichment of Rarely Found Candidatus Anammoxoglobus propionicus from Leachate Sludge
- Source :
- Journal of Microbiology and Biotechnology. 24:879-887
- Publication Year :
- 2014
- Publisher :
- Journal of Microbiology and Biotechnology, 2014.
-
Abstract
- Bacteria that mediate the anaerobic oxidation of ammonium (anammox) have been detected in natural ecosystems, as well as various wastewater treatment systems. In this study, sludge from a particular landfill leachate anaerobic treatment system was selected as the incubation seed for anammox microorganism enrichment owing to its possible anammox activity. Transmission electron microscopy observation, denaturing gradient gel electrophoresis analysis, and cloning/sequencing techniques were applied to identify the diversity of anammox microorganisms throughout the incubation. During the early stage of operation, the diversity of anammox microorganisms was similar to the original complex microbes in the seed sludge. However, as incubation time increased, the anammox microorganism diversity within the system that was originally dominated by Candidatus (Ca.) Brocadia sp. was replaced by Ca. Anammoxoglobus propionicus. The domination of Ca. Anammoxoglobus propionicus produced a stable removal of ammonia (70 mg-N/l) and nitrite (90 mg-N/l), and the total nitrogen removal efficiency was maintained at nearly 95%. The fluorescence in situ hybridization results showed that Ca. Anammoxoglobus propionicus was successfully enriched from 1.8 ± 0.6% initially to 65 ± 5% after 481 days of operation. Therefore, the present results demonstrated the feasibility of enriching Ca. Anammoxoglobus propionicus from leachate sludge, even though the original cell count was extremely low. Application of this seldom found anammox organism could offer an alternative to current ammonia-nitrogen treatment.
- Subjects :
- Bacteriological Techniques
Sewage
biology
Nitrogen
Microorganism
General Medicine
biology.organism_classification
Polymerase Chain Reaction
Applied Microbiology and Biotechnology
Microbiology
Planctomycetales
chemistry.chemical_compound
Microscopy, Electron, Transmission
chemistry
Anammox
Candidatus
Sewage treatment
Ammonium
Food science
Leachate
Phylogeny
Bacteria
Temperature gradient gel electrophoresis
Biotechnology
Subjects
Details
- ISSN :
- 17388872 and 10177825
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....6831fe65102a73afbd2194ce41226d9c
- Full Text :
- https://doi.org/10.4014/jmb.1401.01016