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Tuneable Hybrid Hydrogels via Complementary Self-Assembly of a Bioactive Peptide with a Robust Polysaccharide
- Source :
- ACS Biomaterials Science & Engineering. 7:3340-3350
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly of low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) and Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous and bioactive hydrogels. Polysaccharides such as agarose are biocompatible, degradable, and non-toxic. Agarose and these Fmoc-peptides have both demonstrated efficacy in vitro and in vivo. These materials have complementary properties; agarose has known mechanics in the physiological range but is inert and would benefit from bioactive and topographical cues found in the fibrous, protein-rich extracellular matrix. Fmoc-DIKVAV and Fmoc-FRGDF are synthetic self-assembling peptides that present bioactive cues "IKVAV" and "RGD" designed from the ECM proteins laminin and fibronectin. The work presented here demonstrates that the addition of agarose to Fmoc-DIKVAV and Fmoc-FRGDF results in physical characteristics that are dependent on agarose concentration. The networks are peptide-dominated at low agarose concentrations, and agarose-dominated at high agarose concentrations, resulting in distinct changes in structural morphology. Interestingly, at mid-range agarose concentration, a hybrid network is formed with structural similarities to both peptide and agarose systems, demonstrating reinforced mechanical properties. Bioactive-LMWG polysaccharide hydrogels demonstrate controllable microenvironmental properties, providing the ability for tissue-specific biomaterial design for tissue engineering and 3D cell culture.
- Subjects :
- Phenylalanine
0206 medical engineering
Biomedical Engineering
Biocompatible Materials
Peptide
02 engineering and technology
Biomaterials
Extracellular matrix
3D cell culture
chemistry.chemical_compound
Tissue engineering
Polysaccharides
chemistry.chemical_classification
biology
Hydrogels
021001 nanoscience & nanotechnology
020601 biomedical engineering
Fibronectin
chemistry
Self-healing hydrogels
biology.protein
Biophysics
Agarose
Peptides
0210 nano-technology
Self-assembling peptide
Subjects
Details
- ISSN :
- 23739878
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- ACS Biomaterials Science & Engineering
- Accession number :
- edsair.doi.dedup.....6856016fdc049adf1cd5ec0432aae552