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Adenine nucleotide translocase 3 (ANT3) overexpression induces apoptosis in cultured cells
- Source :
- FEBS letters. 563(1-3)
- Publication Year :
- 2003
-
Abstract
- Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis [Bauer et al. (1999) J. Cell Biol. 439, 258–262]. Nothing is known, however, about the apoptotic activity of ANT3. We have transfected HeLa cells with the three human ANT isoforms to compare their potential as inducers of apoptosis. Transient overexpression of ANT3 resulted, like ANT1, in apoptosis as shown by an increase in the sub-G1 fraction, annexin V staining, low ΔΨm, and activation of caspases 9 and 3. Moreover, the apoptosis produced by ANT3 was inhibited by bongkrekic acid and by cyclosporin A. The pro-apoptotic activities of the ANT1 and ANT3 isoforms contrast with the lack of apoptotic activity of ANT2. This finding may help to identify the specific factors associated with the pro-apoptotic activities of ANT isoforms.
- Subjects :
- Cell death
Programmed cell death
Time Factors
Biophysics
Gene Expression
Apoptosis
Mitochondrion
Transfection
Biochemistry
Membrane Potentials
HeLa
Structural Biology
Annexin
Genetics
Humans
Protein Isoforms
Enzyme Inhibitors
Molecular Biology
Caspase
Adenine nucleotide translocase
biology
Cell Biology
biology.organism_classification
Molecular biology
ANT
Adenine Nucleotide Translocator 3
Cell biology
Mitochondria
Caspases
ADP/ATP carrier
biology.protein
Cyclosporine
ATP–ADP translocase
Bongkrekic Acid
HeLa Cells
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 563
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....68a18795453c4397c963b2b0ae1d0551