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Human organotypic brain slice culture: a novel framework for environmental research in neuro-oncology
- Source :
- Life Science Alliance, Life science alliance 2(4), e201900305 (2019). doi:10.26508/lsa.201900305
- Publication Year :
- 2019
- Publisher :
- Life Science Alliance LLC, 2019.
-
Abstract
- Therapeutically resected, adult brain segments were maintained and characterized for an extended period to study glioblastoma progression and treatment in its almost natural environment.<br />When it comes to the human brain, models that closely mimic in vivo conditions are lacking. Living neuronal tissue is the closest representation of the in vivo human brain outside of a living person. Here, we present a method that can be used to maintain therapeutically resected healthy neuronal tissue for prolonged periods without any discernible changes in tissue vitality, evidenced by immunohistochemistry, genetic expression, and electrophysiology. This method was then used to assess glioblastoma (GBM) progression in its natural environment by microinjection of patient-derived tumor cells into cultured sections. The result closely resembles the pattern of de novo tumor growth and invasion, drug therapy response, and cytokine environment. Reactive transformation of astrocytes, as an example of the cellular nonmalignant tumor environment, can be accurately simulated with transcriptional differences similar to those of astrocytes isolated from acute GBM specimens. In a nutshell, we present a simple method to study GBM in its physiological environment, from which valuable insights can be gained. This technique can lead to further advancements in neuroscience, neuro-oncology, and pharmacotherapy.
- Subjects :
- 0301 basic medicine
Adult
Male
Health, Toxicology and Mutagenesis
medicine.medical_treatment
Plant Science
Biology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Models, Biological
Tissue Culture Techniques
03 medical and health sciences
0302 clinical medicine
Slice preparation
In vivo
Cell Movement
medicine
Temozolomide
Tumor Microenvironment
Humans
Nerve Tissue
Microinjection
Research Articles
Aged
Cell Proliferation
Aged, 80 and over
Tumor microenvironment
Ecology
Cell growth
Brain Neoplasms
fungi
Brain
Infant
Human brain
Middle Aged
030104 developmental biology
Cytokine
medicine.anatomical_structure
030220 oncology & carcinogenesis
Astrocytes
Immunohistochemistry
Female
Glioblastoma
Neuroscience
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 25751077
- Volume :
- 2
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Life Science Alliance
- Accession number :
- edsair.doi.dedup.....68a8a0a86124333dbcc9655f2b030222