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The generally useful estimate of solvent systems method facilitates off‐line two‐dimensional countercurrent chromatography for isolating compositions from Siraitia grosvenorii roots
- Source :
- Journal of Separation Science. 46:2200708
- Publication Year :
- 2023
- Publisher :
- Wiley, 2023.
-
Abstract
- Solvent system selection is a crucial and the most time-consuming step for successful countercurrent chromatography separation. A thin-layer chromatography based generally useful estimate of solvent systems method has been developed to simplify the solvent system selection. We herein utilized the method to select a solvent system for off-line two-dimensional high-performance countercurrent chromatography to separate chemical compositions from a complex fraction of the Siraitia grosvenorii root extract. The first-dimensional countercurrent separation using chloroform/methanol/water (10:5.5:4.5, v/v) yielded four compounds with high purity and three mixture fractions (Fr I, III, and VII). The second-dimensional countercurrent separation conducted on Fr I, III, and VII using the hexane/ethyl acetate/methanol/water (4:6:6:4, 3:7:3:7, v/v) and chloroform/methanol/water (10:9:6, v/v) solvent systems, respectively, produced another four compounds. Four triterpenoids and four lignans were finally isolated, including two novel compounds. Hence, the generally useful estimate of solvent systems method is a feasible and efficient approach for selecting an applicable solvent system for separating complex samples. In addition, the off-line two-dimensional countercurrent chromatography method can improve both the peak resolution and the capacity of countercurrent chromatography. This article is protected by copyright. All rights reserved.
- Subjects :
- Filtration and Separation
Analytical Chemistry
Subjects
Details
- ISSN :
- 16159314 and 16159306
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of Separation Science
- Accession number :
- edsair.doi.dedup.....68afd391ea4247669a0f4c31427e5e4f