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AQP0 is a novel surface marker for deciphering abnormal erythropoiesis
- Source :
- Stem Cell Research & Therapy, Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-10 (2021)
- Publication Year :
- 2020
- Publisher :
- Research Square Platform LLC, 2020.
-
Abstract
- Background Hematopoiesis occurs in the bone marrow, producing a complete spectrum of blood cells to maintain homeostasis. In addition to light microscopy, chromosome analysis, and polymerase chain reaction, flow cytometry is a feasible and fast method for quantitatively analyzing hematological diseases. However, because sufficient specific cell markers are scarce, dyserythropoietic diseases are challenging to identify through flow cytometry. Methods Bone marrow samples from C57BL/B6 mice and one healthy donor were analyzed using traditional two-marker (CD71 and glycophorin A) flow cytometry analysis. After cell sorting, the gene expressions of membrane proteins in early and late erythropoiesis precursors and in nonerythroid cells were characterized using microarray analysis. Results Among characterized gene candidates, aquaporin 0 (AQP0) expressed as a surface protein in early- and late-stage erythropoiesis precursors and was not expressed on nonerythroid cells. With the help of AQP0 staining, we could define up to five stages of erythropoiesis in both mouse and human bone marrow using flow cytometry. In addition, because patients with dyserythropoiesis generally exhibited a reduced population of APQ0high cells relative to healthy participants, the analysis results also suggested that the levels of APQ0high cells in early erythropoiesis serve as a novel biomarker that distinguishes normal from dysregulated erythropoiesis. Conclusions AQP0 was successfully demonstrated to be a marker of erythroid differentiation. The expression levels of AQP0 are downregulated in patients with dyserythropoiesis, indicating a critical role of AQP0 in erythropoiesis. Accordingly, the level of AQP0high in early erythroid precursor cells may serve as a reference parameter for diagnosing diseases associated with dyserythropoiesis.
- Subjects :
- 0301 basic medicine
Medicine (General)
Population
Medicine (miscellaneous)
Aquaporin 0 (AQP0)
Bone Marrow Cells
QD415-436
Biology
Aquaporins
Biochemistry
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Flow cytometry
03 medical and health sciences
Mice
R5-920
0302 clinical medicine
Dyserythropoiesis
medicine
Animals
Humans
Erythropoiesis
Bone marrow
education
Eye Proteins
Erythroid Precursor Cells
education.field_of_study
medicine.diagnostic_test
Research
Cell Biology
Biomarker
Cell sorting
Hematologic Diseases
Cell biology
Hematopoiesis
Mice, Inbred C57BL
Haematopoiesis
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Molecular Medicine
Stem cell
Biomarkers
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy, Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-10 (2021)
- Accession number :
- edsair.doi.dedup.....68c79f017252ac784cdf6be94360cb1d