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Minimal peroxide exposure of neuronal cells induces multifaceted adaptive responses
- Source :
- PLoS ONE, Vol 5, Iss 12, p e14352 (2010), PLoS ONE
- Publication Year :
- 2010
- Publisher :
- Public Library of Science (PLoS), 2010.
-
Abstract
- Oxidative exposure of cells occurs naturally and may be associated with cellular damage and dysfunction. Protracted low level oxidative exposure can induce accumulated cell disruption, affecting multiple cellular functions. Accumulated oxidative exposure has also been proposed as one of the potential hallmarks of the physiological/pathophysiological aging process. We investigated the multifactorial effects of long-term minimal peroxide exposure upon SH-SY5Y neural cells to understand how they respond to the continued presence of oxidative stressors. We show that minimal protracted oxidative stresses induce complex molecular and physiological alterations in cell functionality. Upon chronic exposure to minimal doses of hydrogen peroxide, SH-SY5Y cells displayed a multifactorial response to the stressor. To fully appreciate the peroxide-mediated cellular effects, we assessed these adaptive effects at the genomic, proteomic and cellular signal processing level. Combined analyses of these multiple levels of investigation revealed a complex cellular adaptive response to the protracted peroxide exposure. This adaptive response involved changes in cytoskeletal structure, energy metabolic shifts towards glycolysis and selective alterations in transmembrane receptor activity. Our analyses of the global responses to chronic stressor exposure, at multiple biological levels, revealed a viable neural phenotype in-part reminiscent of aged or damaged neural tissue. Our paradigm indicates how cellular physiology can subtly change in different contexts and potentially aid the appreciation of stress response adaptations.
- Subjects :
- Proteomics
Cell physiology
Aging
Science
Oxidative phosphorylation
Biology
Mitochondrion
Ligands
medicine.disease_cause
Models, Biological
Cell Biology/Cell Signaling
Cell Line, Tumor
medicine
Homeostasis
Humans
Cell Biology/Gene Expression
Neurons
Brain-derived neurotrophic factor
Multidisciplinary
Brain-Derived Neurotrophic Factor
Genomics
Hydrogen Peroxide
Adaptive response
Peroxides
Cell biology
Oxidative Stress
Gene Expression Regulation
Cell Biology/Neuronal and Glial Cell Biology
Cell disruption
Medicine
Calcium
Oxidative stress
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 5
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....68ccbadf76d2e989695f4ccbe3890b7a