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Abnormal expression of circRNA_089763 in the plasma exosomes of patients with post-operative cognitive dysfunction after coronary artery bypass grafting
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2019
- Publisher :
- D.A. Spandidos, 2019.
-
Abstract
- Post-operative cognitive dysfunction (POCD) is a complication of the central nervous system characterized by mental disorders, anxiety, personality changes and impaired memory. POCD occurs frequently after coronary artery bypass grafting (CABG) and can severely affect quality of life for patients. To date, the development of POCD biomarkers remains a challenge. Alterations in the expression of non-coding RNAs from brain tissue and peripheral blood have been linked to POCD. The present study aimed to detect the differential circular RNAs (circRNAs) in plasma exosomes of patients with POCD after CABG. The relative expression levels of circRNAs were analyzed using circRNA microarray analysis in the plasma exosomes of patients with POCD. Differentially altered circRNAs (P1.5) were validated by reverse transcription-quantitative PCR in the plasma exosomes of patients with POCD. The target genes of the microRNAs were predicted using bioinformatics analysis. The functions and signaling pathways of these target genes were investigated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses. The microarray results indicated that the levels of nine circRNAs in patients with POCD were higher than those in the control subjects; and six circRNAs were at a lower level than those in control subjects. The RT-qPCR results from patients with POCD showed that only circRNA_089763 of the 15 circRNAs identified was significantly increased compared with control subjects. circRNA target gene prediction and functional annotation analysis showed significant enrichment in several GO terms and pathways associated with POCD. The present study provides evidence for the abnormal expression of POCD-induced circRNA_089763 in human plasma exosomes, as well as the involvement of POCD.
- Subjects :
- Adult
Male
0301 basic medicine
Cancer Research
Microarray
coronary artery bypass grafting
Gene Expression
exosomes
Bioinformatics
Biochemistry
03 medical and health sciences
post-operative cognitive dysfunction
0302 clinical medicine
microRNA
Genetics
Humans
Medicine
Cognitive Dysfunction
Postoperative Period
Coronary Artery Bypass
KEGG
Molecular Biology
Aged
Aged, 80 and over
Oncogene
business.industry
Microarray analysis techniques
Gene Expression Profiling
Computational Biology
Cancer
circular RNA
RNA, Circular
Articles
Middle Aged
medicine.disease
Molecular medicine
Gene expression profiling
Gene Ontology
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Molecular Medicine
Female
business
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 17913004 and 17912997
- Volume :
- 20
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....68d20e08432a358618403c0ce3a7647f