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Putative model based on iTRAQ proteomics for Spirulina morphogenesis mechanisms

Authors :
Fu-Shuang Wang
Hong-Yu Zhang
Su-Ying Wang
Shi-Rui Dong
Source :
Journal of Proteomics. 171:73-80
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

The morphology of Spirulina during cultivation is susceptible to external interferences, but the morphogenesis mechanism is still unclear. Here the proteomic changes of linear Spirulina and spiral Spirulina were comparatively investigated via isobaric tag for relative and absolute quantitation (iTRAQ). Totally 165 and 167 differences in proteins expression were screened out from the TJSD2/TJSD3 and TJBC4-1/TJBC4-2 groups, respectively. Gene ontology and metabolic pathway analysis of differences in proteins expression uncovered the metabolic pathways (photosynthesis, carbon fixation, sugar metabolism) that were significantly enriched with the proteins correlated with Spirulina morphogenesis. The results of differences in proteins expression in metabolic pathway were verified by quantitative real-time PCR. We also built a putative model of Spirulina morphogenesis mechanism and thought multiple metabolic pathways interact and take part in Spirulina morphogenesis.

Details

ISSN :
18743919
Volume :
171
Database :
OpenAIRE
Journal :
Journal of Proteomics
Accession number :
edsair.doi.dedup.....69162073663df78b407aaec582ff6840
Full Text :
https://doi.org/10.1016/j.jprot.2017.03.017