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Hematopoietic Fingerprints: an expression database of stem cells and their progeny
- Publication Year :
- 2007
-
Abstract
- Hematopoietic stem cells (HSCs) continuously regenerate the hematologic system, yet few genes regulating this process have been defined. To identify candidate factors involved in differentiation and self-renewal, we have generated an expression database of hematopoietic stem cells and their differentiated progeny, including erythrocytes, granulocytes, monocytes, NK cells, activated and naive T cells, and B cells. Bioinformatic analysis revealed HSCs were more transcriptionally active than their progeny and shared a common activation mechanism with T cells. Each cell type also displayed unique biases in the regulation of particular genetic pathways, with Wnt signaling particularly enhanced in HSCs. We identified approximately 100-400 genes uniquely expressed in each cell type, termed lineage "fingerprints." In overexpression studies, two of these genes, Zfp 105 from the NK cell lineage, and Ets2 from the monocyte lineage, were able to significantly influence differentiation toward their respective lineages, demonstrating the utility of the fingerprints for identifying genes that regulate differentiation.
- Subjects :
- Cell type
Genotype
Transcription, Genetic
Cellular differentiation
Biology
computer.software_genre
Article
Cell Line
Proto-Oncogene Protein c-ets-2
03 medical and health sciences
Mice
0302 clinical medicine
Databases, Genetic
Genetics
medicine
Animals
Cell Lineage
030304 developmental biology
Cell Proliferation
0303 health sciences
Database
Monocyte
Gene Expression Profiling
Wnt signaling pathway
Computational Biology
Cell Differentiation
Cell Biology
Chromatin Assembly and Disassembly
Hematopoietic Stem Cells
Molecular biology
STEMCELL
Gene expression profiling
DNA-Binding Proteins
Mice, Inbred C57BL
Wnt Proteins
Haematopoiesis
medicine.anatomical_structure
Phenotype
Gene Expression Regulation
030220 oncology & carcinogenesis
Molecular Medicine
Female
Stem cell
computer
Stem cell lineage database
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....6929cd0a4d18887ce8f5a11309736418