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Generation of Reelin-Positive Marginal Zone Cells from the Caudomedial Wall of Telencephalic Vesicles

Authors :
Mariko Sekiguchi
Yasuto Tanabe
Shizuko Ashigaki
Minesuke Yokoyama
Keiko Takiguchi-Hayashi
Rika Suzuki-Migishima
Hiroshi Hasegawa
Masako Takamatsu
Shigetada Nakanishi
Publication Year :
2004
Publisher :
Society for Neuroscience, 2004.

Abstract

An early and fundamental step of the laminar organization of developing neocortex is controlled by the developmental programs that critically depend on the activities of reelin-positive cells in the marginal zone. However, the ontogeny of reelin-positive cells remained elusive. To gain insights into the spatial and temporal regulation of reelin-positive marginal zone cell development, we used a transgenic mouse line in which we defined the green fluorescent protein (GFP) transgene as a novel reliable molecular marker of reelin-positive marginal zone cells from the early stages of their development. We further usedexo uteroelectroporation-mediated gene transfer that allows us to mark progenitor cells and monitor the descendants in the telencephalonin vivo. We show here the generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles, including the cortical hem, where the prominent expression of GFP is initially detected. These neurons tangentially migrate at the cortical marginal zone and are distributed throughout the entire neocortex in a caudomedial-high to rostrolateral-low gradient during the dynamic developmental period of corticogenesis. Therefore, our findings on reelin-positive marginal zone cells, in addition to the cortical interneurons, add to the emerging view that the neocortex consists of neuronal subtypes that originate from a focal source extrinsic to the neocortex, migrate tangentially into the neocortex, and thereby underlie neural organization of the neocortex.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....693a56e4820f74bbc90e502753c4f108