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Generation of Reelin-Positive Marginal Zone Cells from the Caudomedial Wall of Telencephalic Vesicles
- Publication Year :
- 2004
- Publisher :
- Society for Neuroscience, 2004.
-
Abstract
- An early and fundamental step of the laminar organization of developing neocortex is controlled by the developmental programs that critically depend on the activities of reelin-positive cells in the marginal zone. However, the ontogeny of reelin-positive cells remained elusive. To gain insights into the spatial and temporal regulation of reelin-positive marginal zone cell development, we used a transgenic mouse line in which we defined the green fluorescent protein (GFP) transgene as a novel reliable molecular marker of reelin-positive marginal zone cells from the early stages of their development. We further usedexo uteroelectroporation-mediated gene transfer that allows us to mark progenitor cells and monitor the descendants in the telencephalonin vivo. We show here the generation of reelin-positive marginal zone cells from the caudomedial wall of telencephalic vesicles, including the cortical hem, where the prominent expression of GFP is initially detected. These neurons tangentially migrate at the cortical marginal zone and are distributed throughout the entire neocortex in a caudomedial-high to rostrolateral-low gradient during the dynamic developmental period of corticogenesis. Therefore, our findings on reelin-positive marginal zone cells, in addition to the cortical interneurons, add to the emerging view that the neocortex consists of neuronal subtypes that originate from a focal source extrinsic to the neocortex, migrate tangentially into the neocortex, and thereby underlie neural organization of the neocortex.
- Subjects :
- Telencephalon
Cell Adhesion Molecules, Neuronal
Development/Plasticity/Repair
Green Fluorescent Proteins
Gestational Age
Mice, Transgenic
Neocortex
Nerve Tissue Proteins
Cajal–Retzius cell
Laminar organization
Mice
Cell Movement
Genes, Reporter
mental disorders
medicine
Animals
Genes, Tumor Suppressor
Reelin
Progenitor cell
Cells, Cultured
Neurons
Extracellular Matrix Proteins
Mice, Inbred ICR
biology
Cerebrum
General Neuroscience
Tumor Suppressor Proteins
Serine Endopeptidases
Nuclear Proteins
Tumor Protein p73
Marginal zone
Antigens, Differentiation
DNA-Binding Proteins
Mice, Inbred C57BL
Corticogenesis
Luminescent Proteins
Reelin Protein
medicine.anatomical_structure
Electroporation
nervous system
biology.protein
Neuroscience
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....693a56e4820f74bbc90e502753c4f108