Sources of hepatic triglyceride accumulation during high-fat feeding in the healthy rat

Hepatic triglyceride (HTG) accumulation from peripheral dietary sources and from endogenous de novo lipogenesis (DNL) was quantified in adult Sprague–Dawley rats by combining in vivo localized 1H MRS measurement of total hepatic lipid with a novel ex vivo2H NMR analysis of HTG 2H enrichment from 2H-enriched body water. The methodology for DNL determination needs further validation against standard methodologies. To examine the effect of a high-fat diet on HTG concentrations and sources, animals (n = 5) were given high-fat chow for 35 days. HTG accumulation, measured by in vivo1H MRS, increased significantly after 1 week (3.85 ± 0.60% vs 2.13 ± 0.34% for animals fed on a standard chow diet, P
Funded by: Portuguese Foundation for Science and Technology. Grant Numbers: SFRH/BD/17010/2005, POCTI/QUI/55603/2004 and Portuguese Foundation for Science and Technology/Luso-Espanhola Collaborative Actions. Grant Number: GRICES/CSIC 00620.