Supplemental Methods from Combined Menin and EGFR Inhibitors Synergize to Suppress Colorectal Cancer via EGFR-Independent and Calcium-Mediated Repression of SKP2 Transcription

This supplementary file contains methods for menin immunohistochemistry, reagents, cell culture, proliferation assays, western blotting, plasmids and transfections, RT-PCR, cell visualization assays including clonogenicity, H&E staining, and live cell imaging, ChIP assays, and calcium resin competition assays.